QIAamp MinElute ccfDNA Midi Kit, 50 preps, (5-10 ml)

QIAamp MinElute ccfDNA Midi Kit, 50 preps

QIAamp MinElute ccfDNA Kits greatly simplify concentration and purification of free-circulating DNA from plasma or serum. The procedure can be partially automated on the QIAcube Connect.

Features

• Efficient recovery of fragmented DNA
• High yield ensuring detection of low frequency biomarkers
• Low elution volume in water (20 µl) for high concentration of nucleic acids
• Scalable sample input volume of 1–10 ml
• Removal of contaminants and inhibitors

Performance

The quantity of free-circulating DNA in plasma, serum and other body fluids is usually low, and isolation is a challenge. QIAamp MinElute ccfDNA Kits provide excellent results with efficient purification of high-quality ccfDNA from serum and plasma samples, even when small amounts are present. Compared with other commercially available kits, QIAamp MinElute ccfDNA Kits recovered the greatest amount of ccfDNA.

Procedure

The kits use a fast procedure involving pre-concentration of circulating nucleic acids onto magnetic beads and a cleanup of the resulting pre-eluate on QIAamp MinElute columns in a quick and convenient spin procedure. The robust procedure helps to eliminate sample-to-sample cross-contamination and increases user safety when handling potentially infectious samples.

The simple procedure, which is highly suited for simultaneous processing of multiple samples, provides pure nucleic acids in less than 1 hour for 24 samples. Free-circulating DNA is eluted in Ultra-clean Water, ready for use in downstream reactions or storage a –30 to –15°C. Purified nucleic acids are free of proteins, nucleases and other impurities.

Contents

• QIAamp UCP MinElute Columns: 50
• Collection Tubes 2 ml: 100
• Collection Tubes 1.5 ml: 50
• Ultra-clean Water: 5 x 1.0 ml
• Proteinase K: 2 x 10 ml
• Bead Binding Buffer: 2 x 20 ml
• Magnetic Bead Suspension: 5 x 1.6 ml
• Bead Elution Buffer: 12 ml
• Buffer ACB: 12 ml
• Buffer ACW2: 13 ml
• Bead Elution Tubes: 100