QIAprep Spin Miniprep Kit (250)

For purification of up to 20 μg molecular biology grade plasmid DNA

The QIAprep Spin Miniprep Kit is designed for isolation of up to 20 μg high-purity plasmid or cosmid DNA for use in routine molecular biology applications, including fluorescent and radioactive sequencing and cloning. Even higher yields (up to 30 μg) can be achieved using the High-Yield Supplementary Protocol.

 The QIAprep Spin Miniprep Kit can be automated on the QIAcube Connect.

For optimal results it is recommended to use this product together with QIAvac 24 Plus.

Features

• Ready-to-use plasmid DNA in minutes.
• Reproducible yields of molecular biology grade plasmid DNA.
• Single protocol for high- and low-copy vectors.
• Even higher yields with the High-Yield Supplementary Protocol.
• Improved QIAprep 2.0 Spin Column.
• GelPilot loading dye for convenient sample analysis.

Contents

• QIAprep 2.0 Spin Columns: 250
• Buffer P1: 1 x 20 ml, 1 x 50 ml
• Buffer P2: 1 x 20 ml, 1 x 50 ml
• Buffer N3: 140 ml
• Buffer PB: 150 ml
• Buffer PE (concentrate): 55 ml
• Buffer EB: 55 ml
• LyseBlue®: 1 x 20 µl, 1 x 50 µl
• Loading dye: 550 µl
• RNase A: 1 x 2 mg, 1 x 5 mg
• Collection tubes (2 ml): 250