RNeasy FFPE Kit (50)

For purification of total RNA from formalin-fixed, paraffin-embedded tissue sections

The RNeasy FFPE Kit is specially designed for purifying total RNA from formalin-fixed, paraffin-embedded tissue sections. Special lysis and incubation conditions reverse formaldehye modification of RNA. In addition, the lysis buffer efficiently releases RNA from tissue sections while avoiding further RNA degradation. The kit also uses DNase and DNase Booster Buffer for optimized removal of genomic DNA contamination. RNeasy MinElute spin columns enable purification of total RNA with elution volumes of as low as 10 μl.

Purification can be fully automated on the QIAcube Connect.

Features

• Novel method to overcome formalin crosslinking.
• Efficient release of RNA without compromising integrity.
• Streamlined protocol providing RNA in just 85 minutes.

Contents

• RNeasy MinElute® Spin Columns (pink) (each in a 2 ml Collection Tube): 50
• Collection Tubes (1.5 ml): 50
• Collection Tubes (2 ml): 50
• Buffer RBC: 45 ml
• Buffer PKD: 15 ml
• Proteinase K: 1.25 ml
• RNase-Free DNase I (lyophilized): 1500 units
• RNase-Free Water (for use with RNase-Free DNase I): 1.5 ml
• DNase Booster Buffer: 2 ml
• Buffer RPE (concentrate): 11 ml
• RNase-Free Water: 10 ml