MinElute Gel Extraction Kit (50)

For gel extraction of up to 5 μg DNA fragments (70 bp to 4 kb) in low elution volumes

The MinElute Gel Extraction Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of DNA fragments of 70 bp – 4 kb from up to 400 mg gel slices. The spin columns are designed to allow elution in very small volumes (as little as 10 μl), delivering high yields of highly concentrated DNA. An integrated pH indicator allows easy determination of the optimal pH for DNA binding to the spin column. DNA fragments purified with the MinElute system are ready for direct use in all applications, including sequencing, microarray analysis, ligation and transformation, restriction digestion, labeling, microinjection, PCR, and in vitro transcription.

Features

• Very small elution volumes.
• Fast procedure and easy handling.
• High, reproducible recoveries.
• Gel loading dye for convenient sample analysis.

Contents

• MinElute Spin Columns: 50
• Buffer PB: 30 ml 
• Buffer PE (concentrate): 2 x 6 ml
• Buffer EB: 15 ml
•  pH Indicator: 800 µl 
• Collection Tubes (2 ml): 50
• Loading Dye: 110 µl