Ion Torrent™ Oncomine™ Comprehensive Assay Plus, manual library preparation

The Oncomine Comprehensive Assay Plus is a targeted, next-generation sequencing (NGS) assay that provides a comprehensive genomic profiling solution appropriate for formalin-fixed paraffin-embedded (FFPE) tissues from solid tumors. The assay detects multiple biomarkers associated with targeted and immune checkpoint therapies for cancer research. It allows concurrent analysis of DNA and RNA to simultaneously detect multiple types of variants in a single workflow, including single nucleotide variants (SNVs), insertions and deletions (InDels), copy number variants (CNVs), BRCA1 and BRCA2 large genomic rearrangements (LGRs), genomic instability metric (GIM), microsatellite instability (MSI), and tumor mutational burden (TMB), loss-of-heterozygosity (LOH), and gene fusions featuring FusionSync.

Key features of the assay include:
• Profile 500+ unique genes for single gene and multi-gene biomarker insights
• Enables analysis of various single-gene variants, such as SNVs, indels, fusions, splice variants, and CNV, including both copy number gains and losses 
• Detection of complex biomarkers associated with immunotherapies such as TMB and MSI
• Supports homologous recombination deficiency (HRD) research by detecting mutations in 46 HRR genes, including BRCA LGRs, and assessment genomic scarring with the genomic instability metric (GIM)
• Robust performance from as little as 20 ng of DNA/RNA isolated from FFPE samples including fine needle biopsies
• Content driven by the Oncomine Knowledgebase and experienced scientists helps assure coverage of key targets aligned to published evidence
• Highly automated workflow and streamlined bioinformatics analysis pipeline optimized for Oncomine Comprehensive Assay Plus helps deliver insights